This study focuses on evaluating the consistency between temperature profiles in the neutral atmosphere obtained from Spire and COSMIC-2 RO data, as well as from Advanced Technology Microwave Sounder (ATMS) measurements and radiosonde data. The analysis encompasses Spire and COSMIC-2 wet temperature profiles processed by the University Corporation for Atmospheric Research (UCAR) from September 7, 2021, to October 31, 2022. We compare temperature differences between Spire and COSMIC-2 RO data with collocated NOAA-20 ATMS and RS41 radiosonde measurements. The consistency between Spire and COSMIC-2 data via ATMS and radiosonde measurements is also examined. By carrying out Community Radiative Transfer Model (CRTM) simulations with RO retrievals as inputs, the brightness temperatures (BT) from NOAA-20 ATMS observations are compared with RO-simulated BTs for ATMS CH07 to CH14 and CH19 to CH22 sounding channels over the ocean. The differences between Spire and COSMIC-2 BTs and ATMS observations are less than 0.07 K for CH07-14 and 0.20 K for CH19-22. The study further explores the agreement between Spire and COSMIC-2 to RS41 radiosonde observations (RAOB). Above 0.2 km altitude, RS41 RAOB data agrees well with both Spire and COSMIC-2 temperature profiles, demonstrating a temperature difference below 0.13 K. In addition, we also studied the dependence of the agreement between Spire and COSMIC-2 on latitude intervals, local times, and signal-to-noise ratios (SNRs) by using ATMS data as the reference. Our comparisons demonstrated that the Spire RO data is comparable with COSMIC-2, even though COSMIC-2 has a higher SNR.

