Atmospheric observations (ozone (O3), thermodynamic and dynamic) were carried out at Husbands, Barbados (13.1° N, 59.1° W), Dakar, Senegal (14.75° N 17.49° W) and Cape Verde islands (16.9° N, 24.9° W) using ECCZ ozonesondes and radiosondes from June 12th to September 24th 2010. During this period we were able to successfully sample vertical profiles of tropospheric O3 concentrations associated with the Tropical Wave coming off of the African coast that later resulted in the formation of Tropical Storm Julia on september 12th while the southern portion of the Tropical wave later resulted in the formation of Tropical Storm Mathew over the Eastern Caribbean on September 23rd 2010. High frequency upper air soundings were done every three hours to support the National Science Foundation (NSF) Pre-depression Investigation of Cloud systems in the Tropics (PREDICT) and NASA Genesis and Rapid Intensification Processes (GRIP) projects from 1800UTC on September 20th to 0600UTC on September 22nd 2010. During this period the the wave formed a low on September 22nd followed by the formation of Tropical Storm Mathew at 0300UTC September 23rd 2010. Preliminary results from the high frequency soundings from Barbados, showed that the low developed due to the interaction with the Inter-Tropical Convergent Zone (ITCZ) which created the right atmospheric conditions conducive for the genesis of Tropical Storm Mathew. A detail analysis of the soundngs from all three sites and the role of the ITCZ in the genesis of Tropical Storm Mathew is presented in this work.