This project aims to take NAQFC a step further in the direction of probabilistic AQ prediction by exploring and quantifying the potential value of ensemble predictions of PM2.5, and perturbing three key aspects of PM2.5 modeling: the meteorology, emissions, and CMAQ secondary organic aerosol formulation. This presentation focuses on the impact of meteorological variability, which is represented by three members of NOAA’s Short-Range Ensemble Forecast (SREF) system that were down-selected by hierarchical cluster analysis. These three SREF members provide the physics configurations and initial/boundary conditions for the Weather Research and Forecasting (WRF) model runs that generate required output variables for driving CMAQ that are missing in operational SREF output. We conducted WRF runs for January, April, July, and October 2016 to capture seasonal changes in meteorology. Estimated emissions of trace gases and aerosols via the Sparse Matrix Operator Kernel (SMOKE) system were developed using the WRF output. WRF and SMOKE output drive a 3-member CMAQ mini-ensemble of once-daily, 48-h PM2.5 forecasts for the same four months. The CMAQ mini-ensemble is evaluated against both observations and the current operational deterministic NAQFC products, and analyzed to assess the impact of meteorological biases on PM2.5 variability. Quantification of the PM2.5 prediction uncertainty will prove a key factor to support cost-effective decision-making while protecting public health.