We first compare biases between the satellite and ground-based instruments from the upper stratosphere to the upper mesosphere. We then show a number of time series comparisons at 0.46 hPa, a level that is sensitive to changes in H2O and CH4 entering the stratosphere, but, because almost all CH4 has been oxidized, is relatively insensitive to dynamical variations. Interannual variations and drifts are investigated both with respect to the Aura Microwave Limb Sounder (MLS) (from 2004 onwards), and with respect to each instrument’s climatological mean. We find that the variation in the interannual difference in the mean H2O measured by any two instruments is typically ~1%. Most of the datasets start in, or after, 2004, and show annual increases in H2O of 0-1%/year. In particular, MLS shows a trend of between 0.5%/year and 0.7%/year at the comparison sites. However the two longest measurement datasets used here, with measurements back to 1996, show a much smaller trend of between +0.1%/year and -0.1%/year.